Team

Staff Profiles

Hongkui Zeng, Ph.D.

Executive Director, Structured Science

hongkuiz@alleninstitute.org

Hongkui Zeng joined the Allen Institute for Brain Science in 2006. She currently leads the Structured Science Division to develop and operate high-throughput pipelines to generate large-scale, open-access datasets and tools to accelerate neuroscience discovery. Since joining the Allen Institute, she has also led several research programs, including the Transgenic Technology program, the Human Cortex Gene Survey project, the Allen Mouse Brain Connectivity Atlas project, and the Mouse Cell Types and Connectivity program. Zeng received her Ph.D. in molecular and cell biology from Brandeis University, where she studied the molecular mechanisms of the circadian clock in fruit flies. Then as a postdoctoral fellow at Massachusetts Institute of Technology, she studied the molecular and synaptic mechanisms underlying hippocampus-dependent plasticity and learning. Her current research interests are in understanding neuronal diversity and connectivity in the mouse visual cortical circuit and how different neuronal types work together to process and transform visual information. She has broad scientific experience and a keen interest in using a combined molecular, anatomical and physiological approach to unravel mechanisms of brain circuitry and potential means for treating brain diseases.

Research

Research Interests

The brain circuit is an intricately interconnected network of a vast number of neurons with diverse molecular, anatomical and physiological properties. To understand the principles of information processing in the brain circuit, it is essential to have a systematic understanding of the common and unique properties for each of its components - the neuronal cell types, to monitor their activities while the brain is processing information, and to have the ability to manipulate these neurons to investigate their functions in the brain circuit. Combining genetic tools with large-scale imaging and single-cell analysis technologies presents us with the opportunity to gain systematic understanding of the properties, interconnections and functions of these cell types. My team at the Allen Institute for Brain Science has been building multiple platforms, including single-cell transcriptomics, single and multi-patching electrophysiology, 3D reconstruction of neuronal morphology, high throughput brain-wide connectivity mapping, neuronal activity imaging, and cell type-targeted transgenic and viral to characterize the transcriptomic, physiological, morphological, and connectional properties of different types of neurons in a standardized way, towards a taxonomy of cell types and a description of their connectivity matrix for mouse brain circuits such as the visual cortico-thalamic system. Building such knowledge base lays the foundation towards the understanding of the computational mechanisms of brain circuit function.

Expertise

  • Molecular neuroscience
  • Genetics
  • Neuroanatomy
  • Behavior

Research Programs

  • Cell Types
  • Connectivity
  • Atlasing

Selected Publications View on PUBMED

Sparse recurrent excitatory connectivity in the cortical microcircuit of the adult mouse and human

bioRxiv
April 7, 2018

Seeman SC, Campagnola L, Davoudian PA, Hoggarth A, Hage T, Bosma-Moody A, Baker CA, Lee JH, Mihalas S, Teeter C, Ko AL, Ojemann JG, Gwinn RP, Silbergeld DL, Cobbs C, Phillips J, Lein E, Murphy G, Koch C, Zeng H, Jarsky T

The organization of intracortical connections by layer and cell class in the mouse brain

bioRxiv
April 18, 2018

Harris JA, Mihalas S, Hirokawa KE, Whitesell JD, Knox J, Bernard A, Bohn P, Caldejon S, Casal L, Cho A, Feng D, Gaudreault N, Gerfen C, Graddis N, Groblewski PA, Henry A, Ho A, Howard R, Kuan L, Lecoq J, Luviano J, McConoughey S, Mortrud M, Naeemi M, Ng L, Oh SW, Ouellette B, Sorensen SA, Wakeman W, Wang Q, Williford A, Phillips J, Jones A, Koch C, Zeng H

High resolution data-driven model of the mouse connectome

bioRxiv
April 1, 2018

Knox J, Harris KD, Graddis N, Whitesell JD, Zeng H, Harris JA, Shea-Brown E, Mihalas S

Nontoxic, double-deletion-mutant rabies viral vectors for retrograde targeting of projection neurons

Nature Neuroscience
March 5, 2018

Chatterjee S, Sullivan H, MacLennan BJ, Xu R, Hou Y, Lavin TK, Lea N, Michalski J, Babcock K, Dietrich S, Matthews GA, Beyeler A, Calhoon GG, Glober G, Whitesell J, Yao S, Cetin A, Harris J, Zeng H, Tye K, Reid RC, Wickersham IR

Systematic generation of biophysically detailed models for diverse cortical neuron types

Nature Communications
February 19, 2018

Gouwens NW, Berg J, Feng D, Sorensen SA, Zeng H, Hawrylycz MJ, Koch C, and Arkhipov A

Neuronal cell-type classification: challenges, opportunities and the path forward

Nature Reviews Neuroscience
September 2017

Zeng H, Sanes JR

The BRAIN Initiative Cell Census Consortium: Lessons Learned toward Generating a Comprehensive Brain Cell Atlas

Neuron
November 1, 2017

Ecker JR, Geschwind DH, Kriegstein AR, Ngai J, Osten P, Polioudakis D, Regev A, Sestan N, Wickersham IR, Zeng H

Adult mouse cortical cell taxonomy revealed by single cell transcriptomics

Nature Neuroscience
January 4, 2016

Tasic B, Menon V, Nguyen TN, Kim TK, Jarsky T, Yao Z, Levi B1, Gray LT, Sorensen SA, Dolbeare T, Bertagnolli D, Goldy J, Shapovalova N, Parry S, Lee C, Smith K, Bernard A, Madisen L, Sunkin SM, Hawrylycz M, Koch C, Zeng H

Transgenic mice for intersectional targeting of neural sensors and effectors with high specificity and performance

Neuron
March 4, 2015

Madisen L, Garner AR, Shimaoka D, Chuong AS, Klapoetke NC, Li L, van der Bourg A, Niino Y, Egolf L, Monetti C, Gu H, Mills M, Cheng A, Tasic B, Nguyen TN, Sunkin SM, Benucci A, Nagy A, Miyawaki A, Helmchen F, Empson RM, Knöpfel T, Boyden ES, Reid RC, Carandini M, Zeng H

A mesoscale connectome of the mouse brain

Nature
April 2, 2014

Oh SW, Harris JA, Ng L, Winslow B, Cain N, Mihalas S, Wang Q, Lau C, Kuan L, Henry AM, Mortrud MT, Ouellette B, Nguyen TN, Sorensen SA, Slaughterbeck CR, Wakeman W, Li Y, Feng D, Ho A, Nicholas E, Hirokawa KE, Bohn P, Joines KM, Peng H, Hawrylycz MJ, Phillips JW, Hohmann JG, Wohnoutka P, Gerfen CR, Koch C, Bernard A, Dang C, Jones AR, Zeng H

Genetic approaches to neural circuits in the mouse

Annual Review of Neuroscience
May 17, 2013

Huang JZ & Zeng H

Large-scale cellular-resolution gene profiling in human neocortex reveals species-specific molecular signatures

Cell
April 13, 2012

Zeng H, Shen EH, Hohmann JG, Oh SW, Bernard A, Royall JJ, Glattfelder KJ, Sunkin SM, Morris JA, Guillozet-Bongaarts AL, Smith KA, Ebbert AJ, Swanson B, Kuan L, Page DT, Overly CC, Lein ES, Hawrylycz MJ, Hof PR, Hyde TM, Kleinman JE, Jones AR

A toolbox of Cre-dependent optogenetic transgenic mice for light-induced activation and silencing

Nature Neuroscience
March 25, 2012

Madisen L, Mao T, Koch H, Zhuo J, Berenyi A, Fujisawa S, Hsu YW, Garcia AJ, Gu X, Zanella S, Kidney J, Gu H, Mao Y, Hooks BM, Boyden ES, Buzsáki G, Ramirez JM, Jones AR, Svoboda K, Han X, Turner EE, Zeng H

A robust and high-throughput Cre reporting and characterization system for the whole mouse brain

Nature Neuroscience
December 20, 2010

Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H