Lucas T. Graybuck, Ph.D.
Lucas T. Graybuck (also published as Lucas T. Gray) joined the Allen Institute in 2014 to work in the Mouse Cell Types program under the direction of Bosiljka Tasic. He contributes to projects related to cell type identification, labeling, and epigenetic perturbation for the Mouse Cell Types, Human Genetic Tools, and Epigenetic Control in Transgenic Mice projects.
Before joining the Allen Institute, he worked as a Postdoctoral Fellow in the lab of Dr. Nancy Maizels at the University of Washington. In this position, he studied the roles of G-quadruplex (G4) DNA structures in human gene transcription, and the consequences of disordered expression of G4-unwinding DNA helicases BLM and WRN. His work also explored the genomic binding patterns of general transcription factor TFIIH helicases XPB and XPD, which also interact with G4 DNA.
He earned his Ph.D. in Biochemistry in the lab of Dr. Alan Weiner at the University of Washington where he studied the domesticated piggyBac transposase fusion protein CSB-PGBD3. CSB-PGBD3 is found only in the genomes of simian primates, and appears to be conserved as a transcription factor. His work in the Weiner lab also included transcriptomic analysis of gene expression in cell lines from patients with Cockayne Syndrome, a genetic disorder with both neurodevelopmental and progeria-like symptoms, and conservation analysis of the neural-specific domesticated transposase PGBD5.
Mammalian brain functions are performed by an orchestra of diverse, interconnected cell types, each performing their own role in response to a variety of inputs from other cells. Gene expression determines the functional repertoire of each cell type, and is regulated by transcriptional and epigenetic mechanisms. I am interested in identifying the transcriptional hallmarks of adult neural cell types, in making new genetic tools to label specific cell types, and in perturbing the epigenetic state of specific types of neurons to expand our understanding of the interplay between epigenetic state and neural gene expression.
- Chromatin accessibility
- Data visualization
- Cell Types
Selected Publications View on PUBMED
July 12, 2018
Daigle TL, Madisen L, Hage TA, Valley MT, Knoblich U, Larsen RS, Takeno MM, Huang L, Gu H, Larsen R, Mills M, Bosma-Moody A, Siverts LA, Walker M, Graybuck LT, Yao Z, Fong O, Nguyen TN, Garren E, Lenz GH, Chavarha M, Pendergraft J, Harrington J, Hirokawa KE, Harris JA, Nicovich PR, McGraw MJ, Ollerenshaw DR, Smith KA, Baker CA, Ting JT, Sunkin SM, Lecoq J, Lin MZ2, Boyden ES, Murphy GJ, da Costa NM, Waters J, Li L, Tasic B, Zeng H
March 15, 2018
Rosenberg AB, Roco CM, Muscat RA, Kuchina A, Sample P, Yao Z, Gray L, Peeler DJ, Mukherjee S, Chen W, Pun SH, Sellers DL, Tasic B, Seelig G
January 23, 2017
Gray LT, Yao Z, Nguyen TN, Kim TK, Zeng H, Tasic B
Human Molecular Genetics
May 15, 2016
Tang W, Robles AI, Beyer RP, Gray LT, Nguyen GH, Oshima J, Maizels N, Harris CC, Monnat RJ Jr
January 4, 2016
Tasic B, Menon V, Nguyen TN, Kim TK, Jarsky T, Yao Z, Levi B1, Gray LT, Sorensen SA, Dolbeare T, Bertagnolli D, Goldy J, Shapovalova N, Parry S, Lee C, Smith K, Bernard A, Madisen L, Sunkin SM, Hawrylycz M, Koch C, Zeng H
Nature Chemical Biology
Gray LT, Vallur AC, Eddy J, Maizels N
November 1, 2013
Pavelitz T, Gray LT, Padilla SL, Bailey AD, Weiner AM
Gray LT, Fong KK, Pavelitz T, Weiner AM